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actin a3853  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc actin a3853
    Actin A3853, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 5177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/actin+a3853/pmc13012638-83-64-62?v=Cell+Signaling+Technology+Inc
    Average 97 stars, based on 5177 article reviews
    actin a3853 - by Bioz Stars, 2026-08
    97/100 stars

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    Image Search Results


    Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with Ac-40 actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.

    Journal: The Journal of Biological Chemistry

    Article Title: Actin associates with actively elongating genes and binds directly to the Cdk9 subunit of P-TEFb

    doi: 10.1016/j.jbc.2024.105698

    Figure Lengend Snippet: Cdk9 binds directly to G-actin with a preference towards ATP-actin. A , Western blot of co-immunoprecipitation (IP) experiment for 2Flag-Cdk9 (prey) and 2HA-actin (bait), using empty HA as a control. B , Western blot of co-IP experiment for 2HA-Cdk9 (prey) and indicated Flag/2Flag-tagged actin mutants (baits). Boxed areas cut from the same exposure to remove a construct with weak expression level and a lane with protein ladder. C , Western blot of pulldown assay of LatB-actin using His-Cdk9 as the bait and His-GST as a control bait. His-GST detected with His-HRP antibody, His-Cdk9 with Cdk9 antibody, and LatB-actin with Ac-40 actin antibody. D , NBD-actin monomer-binding assay showing increase in the fluorescence of 0.2 μM NBD-labeled ATP-Mg-G-actin under physiological ionic conditions over a range of concentrations of Cdk9 or known actin-binding protein cofilin. Symbols are data points and lines represent the exponentially fitted binding curves. N = 3, one example presented. Experimentally determined dissociation constants (K D ) calculated according to formula presented in materials and methods. Average K D of three assays is 375 ± 105 nM. E , NBD-actin monomer-binding assay as in ( A ), but using ADP-bound actin. LatB, Latrunculin B.

    Article Snippet: The following antibodies were from Merck: Actin Ac-40 (A3853, 1:1000), Actin Ac-74 (A2228), HRP-conjugated polyHistidine (His-HRP, A7058, 1:5000), HRP-conjugated anti-goat IgG (A9452, 1:5000), HRP-conjugated anti-Flag M2 (A8592, 1:5000), and HRP-conjugated anti-HA (HA-7) (H6533, 1:5000).

    Techniques: Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Construct, Expressing, Binding Assay, Fluorescence, Labeling